CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state CD34 cells are most known for its expression on hematopoietic progenitor cells found in bone marrow and cord blood. While compromising only 1.5% of bone marrow mononuclear cell population, CD34 stem cells are responsible for all lymphohematopoietic lineages and are now commonly used clinically as they have been shown to provide long-term durable donor-derived host lymphohematopoietic reconstitution
Primary human CD34+ cells isolated from umbilical cord blood (CB) mononuclear cells (MNCs) include hematopoietic stem and progenitor cells. CD34+ cells are isolated from cord blood using positive immunomagnetic separation techniques, and citrate-phosphate-dextrose (CPD) is added to the cord blood as an anticoagulant Gating strategy used to identify CD34 positive progenitor cells. Progenitor Cells are identified in the Blast gate by CD45/SSC (A).Cells that are positive for CD34 and HLA‐DR (B) excluding the cells that are positive for CD11b (C) are used for the total CD34 count in a specimen.[Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.
CD34(+) cells and EPC mobilization occurs in HF and shows a biphasic response, with elevation and depression in the early and advanced phases, respectively. This could be related to the myelosuppressive role of TNF-alpha The CD34+ Progenitor Reprogramming Kit contains an integrated set of tools and reagents to enrich, expand and reprogram CD34+ progenitor cells from human peripheral blood samples. Advantages: • Optimized for enrichment, expansion and reprogramming of CD34+ progenitor cells expanded from peripheral blood samples
CD34+ cells are mainly found in the blood, umbilical cord and bone marrow, but are not limited to these tissues. CD34 is also expressed on nonhematopoietic cell types, including muscle satellite cells, keratocytes, interstitial cells, fibrocytes, epithelial progenitors, and endothelial cells CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. CD34+ stem cells are multipotent and can differentiate to all hematopoietic cell types in blood. CD34+ cells can also
CD34 is a glycosylated transmembrane protein and represents a marker for blood and bone marrow-derived progenitor cells, especially for hematopoietic stem cells. Recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state CD34. A membrane-bound cell surface antigen present on and identifying T cells, monocytes, and macrophages. It is found principally on endothelial cells and blood-forming stem cells, and regulates cell-to-cell adhesion. Medical Dictionary, © 2009 Farlex and Partners. Want to thank TFD for its existence Gp38 + CD34 + double positive stromal cells were localized near the crypts in colon (orange-yellow staining in Fig. 1B, Left) and ileum (Fig. 1B, Right) (thereafter called CD34 + CSCs for crypt stromal cells), in close contact with Lgr5 + stem cells (Fig. 1C), and were αSMA -/low (arrows in Fig. 1 D and E, Top; analysis of αSMA expression by intracellular FACS analysis is shown in Fig. S1B) The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:peripheral blood (PBMCs) bone marrow1leukapheresis product2cord blood3differentiated ES and iPS cell lines4,5. | US
A high percentage of CD34 + cells in PBSC products significantly correlated with a short DFS: at a cut-off level of 0.8% CD34 + cells, which is the median percentage of all products, the DFS was. The enumeration of CD34+ cells by flow cytometry is commonly employed to assess progenitor/stem cell numbers in peripheral blood, cord blood, and apheresis products used for peripheral blood stem. CD34 is a cluster of differentiation (CD) first described independently by Civin et al. and Tindle et al.  in a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells CD34 (CD34 Molecule) is a Protein Coding gene. Diseases associated with CD34 include Dermatofibrosarcoma Protuberans and Spindle Cell Lipoma.Among its related pathways are C-MYB transcription factor network and Hematopoietic cell lineage.Gene Ontology (GO) annotations related to this gene include transcription factor binding and sulfate binding The CD34 + cells are isolated, concentrated and formulated and then administered via injection to the same patient. Because the cells are preprogrammed, there is no need for modification, as is the case for CAR-T cell therapies. The expansion and formulation of our cell therapies is achieved using a well-established and well-characterized GMP.
, but CD34+ cells can also differentiate into neural cells (Exp Neurol 2006;197:399) Clinical features In AML, detection of CD34+ cells by flow cytometry and immunohistochemistry are comparable ( Arch Pathol Lab Med 2001;125:1063